In competitive binding assays, 10 mm free synthetic sialic acid (Sigma) was added 30 min before the endocytic agent and left during the assay. These were further purified by size-exclusion chromatography with elution in 50 mm KPi, 200 mm NaCl, pH 7.8, dialyzed against assay buffer (20 mm Tris, 150 ...
To confirm correct sequences in plasmids constructed, the plasmids can be analyzed by standard techniques such as by restriction endonuclease digestion, and/or sequencing according to known methods. Yeast Integrating plasmids (e.g., YIp5) and Yeast Replicating plasmids (the YRp series plasmids) and...
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This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. More specifically, following treatment with neuraminidase from Clostridium perfringens, the PAECs appeared to lose cell-cell contacts, resulting in rather e...
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It was beforehand believed that influenza pandemics occurred at 10- to 14-yr intervals, but it has been over 30 years since H3N2 viruses appeared. Viruses have been first remoted in Hong Kong in July 1968. Widespread disease with increased excess mortality was observed within the United States duri...
As folks discover new methods to get round CAPTCHA, computer scientists like von Ahn develop CAPTCHAs that address other challenges in the sector of AI. In a 2006 lecture, von Ahn talked about the connection between things like CAPTCHA and http://ecotour.ihalla.com the sector of synthetic intellig...
Given the structural resemblance of RgNanOx to YjhC, it is likely that the E. coli oxidoreductase also uses the same mechanism of action for the reversible conversion of 2,7-anhydro-Neu5Ac to Neu5Ac. Given the accessibility of sialic acids in mucus-rich environments, their utilization offers pathog...
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